LOCUS NC_006298 5178 bp DNA circular BCT 27-JUN-2007 DEFINITION Haemophilus somnus 129PT plasmid pHS129, complete sequence. ACCESSION NC_006298 VERSION NC_006298.1 GI:52421262 KEYWORDS . SOURCE Haemophilus somnus 129PT ORGANISM Haemophilus somnus 129PT Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Histophilus. REFERENCE 1 (bases 1 to 5178) AUTHORS Challacombe,J.F., Duncan,A.J., Brettin,T.S., Bruce,D., Chertkov,O., Detter,J.C., Han,C.S., Misra,M., Richardson,P., Tapia,R., Thayer,N., Xie,G. and Inzana,T.J. TITLE Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd JOURNAL J. Bacteriol. 189 (5), 1890-1898 (2007) PUBMED 17172329 REFERENCE 2 (bases 1 to 5178) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (22-SEP-2004) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 5178) CONSRTM Joint Genome Institute Microbial Sequencing, Finishing and Annotation Team TITLE Direct Submission JOURNAL Submitted (02-SEP-2004) Joint Genome Institute, Department of Energy, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000019. Haemophilus somnus strain 129Pt was isolated from the prepuce of a healthy bull at the Washington State Animal Disease Diagnostic Laboratory. This strain lacks several known virulence attributes and is relatively avirulent in challenge models. In general H. somnus is the cause of a variety of systemic diseases in cattle, including thrombotic-meningoencephalitis, pneumonia, abortion and other reproductive diseases, arthritis, myocarditis, and septicemia. Plasmid and fosmid libraries of H.somnus (strain 129Pt) were prepared and shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in WalnutCreek, CA. to a coverage of 6x. Draft reads produced one contig with reads from only plasmid libraries and one gap closed by PCR. Gene predictions were performed using Glimmer and basic analysis was performed by searching the coding sequences against the Pfam, BLOCKS, COGS and PDB databases. Six genes have been annotated and two plasmid replication proteins identified. COMPLETENESS: full length.