LOCUS       NC_006578              77112 bp    DNA     circular BCT 31-JAN-2007
   DEFINITION  Bacillus thuringiensis serovar konkukian str. 97-27 plasmid
               pBT9727, complete sequence.
   ACCESSION   NC_006578
   VERSION     NC_006578.1  GI:56899872
   KEYWORDS    .
   SOURCE      Bacillus thuringiensis serovar konkukian str. 97-27
     ORGANISM  Bacillus thuringiensis serovar konkukian str. 97-27
               Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus
               cereus group.
   REFERENCE   1  (bases 1 to 77112)
     AUTHORS   Han,C.S., Xie,G., Challacombe,J.F., Altherr,M.R., Bhotika,S.S.,
               Brown,N., Bruce,D., Campbell,C.S., Campbell,M.L., Chen,J.,
               Chertkov,O., Cleland,C., Dimitrijevic,M., Doggett,N.A.,
               Fawcett,J.J., Glavina,T., Goodwin,L.A., Green,L.D., Hill,K.K.,
               Hitchcock,P., Jackson,P.J., Keim,P., Kewalramani,A.R., Longmire,J.,
               Lucas,S., Malfatti,S., McMurry,K., Meincke,L.J., Misra,M.,
               Moseman,B.L., Mundt,M., Munk,A.C., Okinaka,R.T.,
               Parson-Quintana,B., Reilly,L.P., Richardson,P., Robinson,D.L.,
               Rubin,E., Saunders,E., Tapia,R., Tesmer,J.G., Thayer,N.,
               Thompson,L.S., Tice,H., Ticknor,L.O., Wills,P.L., Brettin,T.S. and
     TITLE     Pathogenomic sequence analysis of Bacillus cereus and Bacillus
               thuringiensis isolates closely related to Bacillus anthracis
     JOURNAL   J. Bacteriol. 188 (9), 3382-3390 (2006)
      PUBMED   16621833
     REMARK    Erratum:[J Bacteriol. 2006 Nov;188(21):7711. Brown, Nancy [added];
               Green, Lance D [added]]
   REFERENCE   2  (bases 1 to 77112)
     CONSRTM   Joint Genome Institute Microbial Sequencing, Finishing and
               Annotation Team
     TITLE     A 77 kb plasmid sequence of Bacillus thuringiensis 97-27
     JOURNAL   Unpublished
   REFERENCE   3  (bases 1 to 77112)
     CONSRTM   NCBI Genome Project
     TITLE     Direct Submission
     JOURNAL   Submitted (30-DEC-2004) National Center for Biotechnology
               Information, NIH, Bethesda, MD 20894, USA
   REFERENCE   4  (bases 1 to 77112)
     CONSRTM   Joint Genome Institute Microbial Sequencing, Finishing and
               Annotation Team
     TITLE     Direct Submission
     JOURNAL   Submitted (15-DEC-2004) Joint Genome Institute, Department of
               Energy, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
   COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
               NCBI review. The reference sequence was derived from CP000047.
               Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was
               originally isolated from a case of severe human tissue necrosis
               (Bacillus thuringiensis subsp. konkukian (serotype H34)
               superinfection: Case report and experimental evidence of
               pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F,
               Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998
               36(7):2138-2139). B. thuringiensis is indigenous to many habitats
               worldwide; these include soil, insects, deciduous and coniferous
               leaves (Prediction of insecticidal activity of Bacillus
               thuringiensis strains by polymerase chain reaction product
               profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and
               Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B.
               thuringiensis is an insect pathogen that is widely used as a
               biopesticide in commercial agriculture. Infection of humans is
               unusual. The apparent pathogenic properties of B. thuringiensis
               97-27 are very unusual for B. thuringiensis; unlike most B.
               thuringiensis isolates, this isolate is very closely related to B.
               anthracis based on phylogenetic analysis (Fluorescent amplified
               fragment length polymorphism analysis of Bacillus anthracis,
               Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK,
               Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M,
               Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD,
               Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ
               Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were
               prepared at the Joint Genome Institute in Los Alamos (JGI-LANL),
               NM. Shotgun sequencing was performed at the JGI Production Genomics
               Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. Gene
               predictions were obtained using Glimmer. Basic analysis of the gene
               predictions was performed by comparing coding sequences against the
               PFam, COGS, BLOCKS and Prodom databases. Gene definitions and
               functional classes were added manually by a team of annotators at
               JGI-LANL, using BLAST results in addition to information from the
               basic analysis. A total of 80 genes have been annotated on the
               sequence record.
               COMPLETENESS: full length.